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One-carbon bound THF exists in different oxidation states (5,10-methylene tetrahydrofolate (5,10-meTHF), 5-methyl-THF, formate (10-formyl THF)), and supports distinct biosynthetic functions, for example, 5,10-meTHF for pyrimidine biosynthesis and 5-methyl-THF for purine biosynthesis 59